RBFOX3/NeuN Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-10491

Novus Biologicals, part of Bio-Techne
Discontinued Product
NBP2-10491 has been discontinued. View all RBFOX3/NeuN products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen (Negative), Immunohistochemistry-Paraffin

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Format

BSA Free

Concentration

0.1 mg/ml

View all RBFOX3/NeuN Antibodies »

Product Specifications

Immunogen

Hens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to RBFOX3/NeuN, but was shared between the human NP_001076044, NCBI) and mouse (NP_001020102, NCBI) sequences.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33754020). Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%).

Marker

Neuron Cell Marker

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

33.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RBFOX3/NeuN Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody [NBP2-10491]

Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody [NBP2-10491]

Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody [NBP2-10491] - Immunohistochemical photomicrograph of adult mouse hippocampal formation stained for beta-III Tubulin rabbit antibody (GREEN) and RBFOX3/NeuN chicken antibody (RED).
Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody [NBP2-10491]

Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody [NBP2-10491]

Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody [NBP2-10491] - Immunohistochemical photomicrograph of adult mouse cerebral cortex stained for beta-III Tubulin rabbit antibody (GREEN) and RBFOX3/NeuN chicken antibody (RED).
Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody [NBP2-10491]

Immunocytochemistry/ Immunofluorescence: RBFOX3/NeuN Antibody [NBP2-10491]

Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody [NBP2-10491] - Immunohistochemical photomicrograph of adult mouse cerebral cortex stained for beta-III Tubulin rabbit antibody (GREEN) and RBFOX3/NeuN chicken antibody (RED).
View 3 more images

Applications for RBFOX3/NeuN Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000-1:2000

Immunohistochemistry

1:1000 - 1:2000

Immunohistochemistry-Paraffin

1:1000 - 1:2000
Application Notes
Use in Flow reported in scientific literature (PMID:33754020).

IHC-Fr is not recommended for use as multiple customers have reported lack of signal using this antibody in frozen sections

Reviewed Applications

Read 1 review rated 1 using NBP2-10491 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

10 mM PBS (0.9% isotonic, w/v, pH 7.2).

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: RBFOX3/NeuN

Rbfox3 (also known as Fox-3, Fox1 homolog C, Hrnbp3, and D11Bwg0517e) is one of three mammalian members of the RNA-binding protein Rbfox gene family, all of which are involved in regulating alternative RNA splicing. Originally identified as an epitope, NeuN is located within the N-terminal region (6-15 amino acids) of Rbfox3. Rbfox family members are highly conserved, having a single RNA recognition motif (RRM)-type RNA binding domain (RBD) near the center of the protein sequence. The RBD amino acid (aa) sequence is identical between members, Rbfox1 and Rbfox2, with 4 differences within the 77 aa domain of Rbfox3. The fox3 gene is comprised of 15 exons in humans, 11 exons in rat, with 3 variants in mouse containing 14 exons and another 3 variants containing 15 exons. The Rbfox3 protein is highly conserved across human, mouse, and rat with 98.9% sequence identity between mouse (isoform I) and rat and 83.9% sequence identity between mouse (isoform I) and human. The expression of Rbfox3/NeuN is restricted to the nervous system and has widely been used in stroke research. It is recognized as a marker of mature neuronal cell types in the spinal cord, cerebral cortex, hippocampus, dorsal thalamus, caudate/putamen, and cerebellum. NeuN has been shown to bind DNA and is predominantly nuclear. Differences in immunoreactivity have been reported between Rbfox3/NeuN subtypes in which the 46kDa is mainly found in the nucleus whereas the 48kDa form is primarily distributed in the cytoplasm (1,2).

Rbfox proteins participate in regulation of alternative splicing between family members as well as in autoregulation. While the breadth of brain and muscle specific targets for alternative splicing is well established for Rbfox proteins, those specific to Rbfox3/NeuN along with its alternative splicing mechanism are less understood. Rbfox3 has been shown to regulate neuronal differentiation by alternative splicing of Numb pre-mRNA and has a role in adult neurogenesis. Dysfunctional Rbfox3/NeuN has been associated with various neurological disorders such as neurodevelopmental delay, autism spectrum disorder, Benign rolandic epilepsy (BRE), and cognitive impairments (1,2).

References

1. Duan W, Zhang YP, Hou Z, Huang C, Zhu H, Zhang CQ, Yin Q. (2016) Novel Insights into NeuN: from Neuronal Marker to Splicing Regulator. Mol Neurobiol. 53(3):1637-1647. PMID: 25680637.

2. Su CH, D D, Tarn WY. (2018) Alternative Splicing in Neurogenesis and Brain Development. Front Mol Biosci. 5:12. PMID: 29484299

Long Name

RNA binding fox-1 homolog 3

Alternate Names

FOX-3, FOX3, HRNBP3, NEUN

Gene Symbol

RBFOX3

UniProt

NP_001076044 (Human), NP_001020102 (Mouse)

Additional RBFOX3/NeuN Products

Product Documents for RBFOX3/NeuN Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RBFOX3/NeuN Antibody - BSA Free

Chicken products cannot be exported to Canada.

Purification Notes
After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation filter-sterilized.

Storage Notes
Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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