RBM10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84951

Novus Biologicals, part of Bio-Techne
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NBP1-84951
NBP1-84951-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RBM10 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MEYERRGGRGDRTGRYGATDRSQDDGGENRSRDHDYRDMDYRSYPREYGSQEGKHDYDDSSEEQSAEDSYEASPGSETQRR

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25586139).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RBM10 Antibody

Western Blot: RBM10 Antibody [NBP1-84951]

Western Blot: RBM10 Antibody [NBP1-84951]

Western Blot: RBM10 Antibody [NBP1-84951] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-RBM10 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: RBM10 Antibody [NBP1-84951]

Immunocytochemistry/ Immunofluorescence: RBM10 Antibody [NBP1-84951]

Immunocytochemistry/Immunofluorescence: RBM10 Antibody [NBP1-84951] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RBM10 Antibody [NBP1-84951]

Immunohistochemistry-Paraffin: RBM10 Antibody [NBP1-84951]

Immunohistochemistry-Paraffin: RBM10 Antibody [NBP1-84951] - Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.
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Applications for RBM10 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBM10

RBM10 contains an RNA recognition motif found in a variety of RNA binding proteins, including various hnRNP proteins, proteins implicated in regulation of alternative splicing, and protein components of snRNPs.

Alternate Names

DXS8237ERNA-binding motif protein 10, G patch domain-containing protein 9, GPATC9TARPS, GPATCH9MGC1132, KIAA0122RNA-binding protein S1-1, MGC997, RNA binding motif protein 10, RNA-binding protein 10, S1-1, ZRANB5

Gene Symbol

RBM10

Additional RBM10 Products

Product Documents for RBM10 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RBM10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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