RBM34 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97638

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RBM34 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human RBM34 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for RBM34 Antibody

Western Blot: RBM34 Antibody [NBP2-97638]

Western Blot: RBM34 Antibody [NBP2-97638]

Western Blot: RBM34 Antibody [NBP2-97638] - Lane A: Hela Whole Cell LysateLane B: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:49 kDaObserved band size:55 kDa
Immunocytochemistry/ Immunofluorescence: RBM34 Antibody [NBP2-97638]

Immunocytochemistry/ Immunofluorescence: RBM34 Antibody [NBP2-97638]

Immunocytochemistry/Immunofluorescence: RBM34 Antibody [NBP2-97638] - Immunofluorescence staining of RBM34 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human RBM34 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Nucleolus.
Immunoprecipitation: RBM34 Antibody [NBP2-97638]

Immunoprecipitation: RBM34 Antibody [NBP2-97638]

Immunoprecipitation: RBM34 Antibody [NBP2-97638] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-RBM34 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-RBM34 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 49 kDaObserved band size :56 kDa

Applications for RBM34 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBM34

RNA binding motif protein 34 (RBM34) is an RRM (RNA recognition motif)-containing protein.

Alternate Names

KIAA0117RNA-binding motif protein 34, RNA binding motif protein 34, RNA-binding protein 34

Gene Symbol

RBM34

Additional RBM34 Products

Product Documents for RBM34 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RBM34 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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