RBM8A Antibody (PCRP-RBM8A-1B4) [mFluor Violet 610 SE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08800MFV610

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array, Western Blot

Label

mFluor Violet 610 SE (Excitation = 421 nm, Emission = 613 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-RBM8A-1B4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RBM8A Antibodies »

Product Summary for RBM8A Antibody (PCRP-RBM8A-1B4) [mFluor Violet 610 SE]

Immunogen

Recombinant full-length human RBM8A protein (Uniprot: Q9Y5S9)

Localization

Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein.

Specificity

RBM8A Antibody is a high quality monoclonal RBM8A antibody (also designated RBM8A antibody) suitable for the detection of the RBM8A protein of mouse, rat, human and Xenopus laevis origin. RBM8A Antibody (4C4) is available as both the non-conjugated anti-RBM8A antibody form, as well as multiple conjugated forms of anti-RBM8A antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor conjugates. The exon junction complex (EJC) is a multiprotein complex that assembles approximately 20-24 nucleotides upstream of exon-exon junctions in pre-mRNAs. It is involved in mRNA export, cytoplasmic localization, and nonsense-mediated mRNA decay. Members of the EJC include RBM8A, Aly/REF, Magoh, RNPS1, SRm160, and DEK. Aly/REF, Magoh, and RBM8A, identified as RBM8 in mouse and rat, make up the core of the EJC, and these proteins remain stably bound to spliced mRNAs in the cytoplasm until they are translated. Therefore, RBM8A, Aly/REF, and Magoh have the ability to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. The gene encoding human RBM8A encodes three transcripts. RBM8A is a ubiquitously expressed protein. Although RBM8A shuttles to the cytoplasm, it is predominantly detected in the nucleus and is co-localized with oskar mRNA at the posterior pole of the cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for RBM8A Antibody (PCRP-RBM8A-1B4) [mFluor Violet 610 SE]

RBM8A Antibody (PCRP-RBM8A-1B4) [mFluor Violet 610 SE]

RBM8A Antibody (PCRP-RBM8A-1B4) [mFluor Violet 610 SE] [NBP3-08800MFV610] -

RBM8A Antibody (PCRP-RBM8A-1B4) [mFluor Violet 610 SE] [NBP3-08800MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission maximum of 613 nm.

Applications for RBM8A Antibody (PCRP-RBM8A-1B4) [mFluor Violet 610 SE]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Protein Array

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: RBM8A

Pre-mRNA splicing appears to play an important role in regulation of gene expression. It has been shown in a number of studies that during the splicing process, specific proteins are recruited to the mRNA and assemble into a complex of mRNA-protein near the exon-exon junction. This complex is termed mRNA-protein complex (mRNP), or the exon-exon junction complex. Several proteins were found to be present in this complex including Y14, magoh, Aly/REF, RNPS1, Upf3, and DEK. Y14 is a 174 amino acid RNA-binding protein that contains one RNA-binding domain in the middle of the protein. Y14 binds to the spliced mRNAs in the nucleus and is exported with the mRNA to the cytoplasm. Similar to other proteins in the mRNA-protein complex, Y14 can interact with the mRNA export factor TAP and enhance the export of the mRNAs. Y14 binds to different mRNAs approx.

Alternate Names

Binder of OVCA1-1, BOV-1, BOV-1A, BOV-1B, BOV-1C, MDS014, RBM8BRBM8, ribonucleoprotein RBM8, Ribonucleoprotein RBM8A, RNA binding motif protein 8A, RNA binding motif protein 8B, RNA-binding motif protein 8A, RNA-binding protein 8A, RNA-binding protein Y14, Y14, ZNRP, ZRNP1

Gene Symbol

RBM8A

Additional RBM8A Products

Product Documents for RBM8A Antibody (PCRP-RBM8A-1B4) [mFluor Violet 610 SE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RBM8A Antibody (PCRP-RBM8A-1B4) [mFluor Violet 610 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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