RBP4/Retinol-Binding Protein 4 Antibody (RB42)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05426
![Western Blot: RBP4/Retinol-Binding Protein 4 Antibody (RB42) [NBP1-05426]](https://resources.bio-techne.com/images/products/RBP4-Retinol-Binding-Protein-4-Antibody-RB42-Western-Blot-NBP1-05426-img0009.jpg "Western Blot: RBP4/Retinol-Binding Protein 4 Antibody (RB42) [NBP1-05426]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # RB42
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for RBP4/Retinol-Binding Protein 4 Antibody (RB42)
Immunogen
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Human recombinant RBP4.
Specificity
Human plasma RBP4. All MAbs recognize free RBP4 and RBP4 in complex with transthyretin. All MAbs react with apo- as well as holo-RBP4.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for RBP4/Retinol-Binding Protein 4 Antibody (RB42)
Western Blot: RBP4/Retinol-Binding Protein 4 Antibody (RB42) [NBP1-05426]
Western Blot: RBP4/Retinol-Binding Protein 4 Antibody (RB42) [NBP1-05426] - Immunodetection of RBP4 in Western blotting after SDSelectrophoresis in reducing conditions by MAb RB42 (Cat. NBP1-05426) (lane 1), RB45 (Cat. NBP1-105431) (lane 2), RB48 (Cat. NBP1-105430) (lane 3) and RB51 (Cat.NBP1-105428) (lane 4).
Sandwich ELISA: RBP4/Retinol-Binding Protein 4 Antibody (RB42) [NBP1-05426] - Immunodetection of purified endogenous RBP4 in sandwich immunoassay by RB48-RB42 MAb assay in presence of 5 mM EDTA (blue line) or in absence of EDTA (pink line). Capture antibody: RB48 (Cat NBP1-105430) (1 ug/well) Detection antibody: RB42 (Cat NBP1-05426) labeled with stable Eu3+ chelate (0.2ug/well) Antigen: Native isolated RBP4
Sandwich ELISA: RBP4/Retinol-Binding Protein 4 Antibody (RB42) [NBP1-05426] - Immunoreactivity of native free and native complexed RBP4 after several freeze-thaw cycles measured with the assay RB48 - RB42. Capture antibody: RB48 (Cat. NBP1-105430) (1 ug/well) Detection antibody: RB42 (Cat NBP1-05426) labeled with stable Eu3+ chelate (0.2ug/well) Antigen: Native isolated RBP4.
Applications for RBP4/Retinol-Binding Protein 4 Antibody (RB42)
Application
Recommended Usage
Sandwich ELISA
0.2 ug/well
Western Blot
1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RBP4/Retinol-Binding Protein 4
Additional RBP4/Retinol-Binding Protein 4 Products
Product Documents for RBP4/Retinol-Binding Protein 4 Antibody (RB42)
Product Specific Notices for RBP4/Retinol-Binding Protein 4 Antibody (RB42)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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