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RBPMS Antibody (OTI3B7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73836

Novus Biologicals, part of Bio-Techne
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NBP2-73836

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3B7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human RBPMS (NP_001008712) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

24.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RBPMS Antibody (OTI3B7) - Azide and BSA Free

Western Blot: RBPMS Antibody (OTI3B7)Azide and BSA Free [NBP2-73836]

Western Blot: RBPMS Antibody (OTI3B7)Azide and BSA Free [NBP2-73836]

Western Blot: RBPMS Antibody (OTI3B7) - Azide and BSA Free [NBP2-73836] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RBPMS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBPMS.
Immunohistochemistry: RBPMS Antibody (OTI3B7) - Azide and BSA Free [NBP2-73836]

Immunohistochemistry: RBPMS Antibody (OTI3B7) - Azide and BSA Free [NBP2-73836]

Immunohistochemistry: RBPMS Antibody (OTI3B7) - Azide and BSA Free [NBP2-73836] - Staining of paraffin-embedded Human pancreas tissue using anti-RBPMS mouse monoclonal antibody.
Flow Cytometry: RBPMS Antibody (OTI3B7) - Azide and BSA Free [NBP2-73836]

Flow Cytometry: RBPMS Antibody (OTI3B7) - Azide and BSA Free [NBP2-73836]

Flow Cytometry: RBPMS Antibody (OTI3B7) - Azide and BSA Free [NBP2-73836] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RBPMS antibody, and then analyzed by flow cytometry.

Applications for RBPMS Antibody (OTI3B7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RBPMS

Encoded by a member of the RRM family of RNA-binding proteins, RBPMS is involved in nucleic acid, nucleotide, RNA and protein binding. The RRM family can be found between 80-100 amino acids in length. RBPMS also acts as a coactivator of transcriptional activity and is required for TGFB1/Smad-mediated transactivation. More specifically, RBPMS acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity while promoting the nuclear accumulation of these proteins. Diseases associated with RBPMS include hepatocellular carcinoma, ataxia, carcinoma and neuronitis.

Alternate Names

FLJ32971, Heart and RRM expressed sequence, Hermes, HERMESRNA-binding protein with multiple splicing, RBP-MS, RNA binding protein with multiple splicing

Gene Symbol

RBPMS

Additional RBPMS Products

Product Documents for RBPMS Antibody (OTI3B7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RBPMS Antibody (OTI3B7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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