RC74 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-58713

Novus Biologicals, part of Bio-Techne
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NBP2-58713
NBP2-58713-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RC74 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: FAEWLCHNKQSKVYLPEPPFPHAELIQTNKLKHYPSIHGDFSNDFRQPCVVFTGHPSLRFGDVVHFMELWGKSSLNTVIFTEPDFSYLEALAP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RC74 Antibody

Western Blot: RC74 Antibody [NBP2-58713]

Western Blot: RC74 Antibody [NBP2-58713]

Western Blot: RC74 Antibody [NBP2-58713] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: RC74 Antibody [NBP2-58713]

Immunocytochemistry/ Immunofluorescence: RC74 Antibody [NBP2-58713]

Immunocytochemistry/Immunofluorescence: RC74 Antibody [NBP2-58713] - Staining of human cell line U-2 OS shows localization to nucleoplasm.

Applications for RC74 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RC74

Formation of the mature 3' ends of the vast majority of cellular mRNAs occurs through cleavage and polyadenylation and requires a cleavage and polyadenylation specificity factor (CPSF) containing, among other proteins, CPSF-73 and CPSF-100. RC-74 is an exclusively nuclear protein found in HeLa cells that has similar characteristics to CPSF-100. Like the complex that forms between CPSF-100 and CPSF-73, studies show that RC-74 may form a similar complex with RC-68 that is independent from the CPSF complex. This RC-68 / RC-74 complex may be involved in a pre-mRNA processing event other than cleavage and polyadenylation, such as the 3' end processing of a distinct subset of cellular pre-mRNAs encoding proteins required for G(1) progression and entry into S phase.

Alternate Names

CPSF2L, FLJ10871, Int9, integrator complex subunit 9, Protein related to CPSF subunits of 74 kDa, RC74, RC-74

Gene Symbol

INTS9

Additional RC74 Products

Product Documents for RC74 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RC74 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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