RDH5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52047
Key Product Details
Species Reactivity
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-RDRQSLPASNAFV corresponding to internal region according to NP_002896.2.
Epitope
C-RDRQSLPASNAFV
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for RDH5 Antibody
Immunohistochemistry-Paraffin: RDH5 Antibody [NBP1-52047] -
Immunohistochemistry-Paraffin: RDH5 Antibody [NBP1-52047] - (5ug/ml) staining of paraffin embedded Human Retina. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunocytochemistry/Immunofluorescence: RDH5 Antibody [NBP1-52047] -
Immunocytochemistry/Immunofluorescence: RDH5 Antibody [NBP1-52047] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong Endoplasmic Reticulum and weak cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: RDH5 Antibody [NBP1-52047] -
Flow Cytometry: RDH5 Antibody [NBP1-52047] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for RDH5 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry
5-10 ug/ml
Immunohistochemistry-Paraffin
5-10 ug/ml
Peptide ELISA
Detection limit 1:8000
Application Notes
WB: Preliminary testing showed a band at approx. 32kDa in Human Heart and approx. 35kDa in Human Breast lysate, after 0.3ug/ml antibody staining. (calculated MW of 35.0kDa according to NP_002896.2). Primary incubation 1 hour at room temperature. IHC-P: Human Retina shows staining of the ganglion cell layer.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RDH5
Alternate Names
11-cis RDH, 11-cis retinol dehydrogenase, 9,9-cis-retinol specific dehydrogenase, EC 1.1.1, EC 1.1.1.105, FLJ39337, FLJ97089, HSD17B, RDH1, retinol dehydrogenase 1, retinol dehydrogenase 5 (11-cis and 9-cis), retinol dehydrogenase 5 (11-cis/9-cis), SDR9C5, short chain dehydrogenase/reductase family 9C, member 5
Gene Symbol
RDH5
UniProt
Additional RDH5 Products
Product Documents for RDH5 Antibody
Product Specific Notices for RDH5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...