Key Product Details

Species Reactivity

Validated:

Rat

Applications

Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen

Label

Janelia Fluor 635

Antibody Source

Monoclonal Mouse IgG1 Clone # HIS52

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RecA-1 Antibodies »

Product Summary for RecA-1 Antibody (HIS52) [Janelia Fluor® 635]

Immunogen

Stromal cells from rat lymph node

Reactivity Notes

Does not react with:Goat, Chicken, Guinea Pig, Sheep, Mouse, Rabbit, Pig

Specificity

Recognizes RECA-1, a cell surface antigen which is expressed by all rat endothelial cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for RecA-1 Antibody (HIS52) [Janelia Fluor® 635]

Application
Recommended Usage

Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: RecA-1

RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognizes a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell specific antigens.

Alternate Names

RecA protein, RecA1, RecA2, recaLP, Recombinase A

Additional RecA-1 Products

Product Documents for RecA-1 Antibody (HIS52) [Janelia Fluor® 635]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RecA-1 Antibody (HIS52) [Janelia Fluor® 635]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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