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Reelin Antibody (142) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1080

Novus Biologicals, part of Bio-Techne
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NB600-1080

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 142

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Recombinant reelin amino acids 40-189.

Reactivity Notes

React in most vertebrates. Rat reactivity reported in scientific literature (PMID: 22252605). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Secreted

Specificity

Reelin. The epitope is between amino acids 164 and 189.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Reelin Antibody (142) - BSA Free

Immunohistochemistry: Reelin Antibody (142) - BSA Free [NB600-1080]

Immunohistochemistry: Reelin Antibody (142) - BSA Free [NB600-1080]

Immunohistochemistry: Reelin Antibody (142) [NB600-1080] - Mouse anti-Reelin staining of Reelin-positive Cajal-Retzius neurons in a human fetal cerebral cortex at 21 weeks of gestation, using anti-Reelin MAB5366 (dilution 1:500). Photo courtesy of Dr. G. Meyer.
Immunohistochemistry: Reelin Antibody (142) - BSA Free [NB600-1080]

Immunohistochemistry: Reelin Antibody (142) - BSA Free [NB600-1080]

Immunohistochemistry: Reelin Antibody (142) [NB600-1080] - Mouse anti-Reelin visualization of a Cajal-Retzius neuron from a human newborn stained for reelin using MAB5366. This photo is courtesy of Dr. G. Meyer.

Applications for Reelin Antibody (142) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:250-1:500

Western Blot

1:250-1:500
Application Notes
Western Blot: Reelin can appear as three bands ~400 - 450, 300, and 180 - 200 kDa's.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

20mM Phosphate buffer and 0.25M NaCl

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Reelin

The Reelin gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorde

Alternate Names

Reeler, RELN, RL

Entrez Gene IDs

5649 (Human)

Gene Symbol

RELN

UniProt

Additional Reelin Products

Product Documents for Reelin Antibody (142) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Reelin Antibody (142) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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