Reelin Antibody (142) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1080
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 142
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant reelin amino acids 40-189.
Reactivity Notes
React in most vertebrates. Rat reactivity reported in scientific literature (PMID: 22252605). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Secreted
Specificity
Reelin. The epitope is between amino acids 164 and 189.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Reelin Antibody (142) - BSA Free
![Immunohistochemistry: Reelin Antibody (142) - BSA Free [NB600-1080]](https://resources.bio-techne.com/images/products/Reelin-Antibody-142-Immunohistochemistry-NB600-1080-img0008.jpg "Immunohistochemistry: Reelin Antibody (142) - BSA Free [NB600-1080]")
Immunohistochemistry: Reelin Antibody (142) - BSA Free [NB600-1080]
Immunohistochemistry: Reelin Antibody (142) [NB600-1080] - Mouse anti-Reelin staining of Reelin-positive Cajal-Retzius neurons in a human fetal cerebral cortex at 21 weeks of gestation, using anti-Reelin MAB5366 (dilution 1:500). Photo courtesy of Dr. G. Meyer.![Immunohistochemistry: Reelin Antibody (142) - BSA Free [NB600-1080]](https://resources.bio-techne.com/images/products/Reelin-Antibody-142-Immunohistochemistry-NB600-1080-img0006.jpg "Immunohistochemistry: Reelin Antibody (142) - BSA Free [NB600-1080]")
Immunohistochemistry: Reelin Antibody (142) - BSA Free [NB600-1080]
Immunohistochemistry: Reelin Antibody (142) [NB600-1080] - Mouse anti-Reelin visualization of a Cajal-Retzius neuron from a human newborn stained for reelin using MAB5366. This photo is courtesy of Dr. G. Meyer.Applications for Reelin Antibody (142) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:250-1:500
Western Blot
1:250-1:500
Application Notes
Western Blot: Reelin can appear as three bands ~400 - 450, 300, and 180 - 200 kDa's.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
20mM Phosphate buffer and 0.25M NaCl
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Reelin
Additional Reelin Products
Product Documents for Reelin Antibody (142) - BSA Free
Product Specific Notices for Reelin Antibody (142) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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