Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Flow Cytometry Control, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 112A1021

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all RelA/NFkB p65 Antibodies »

Product Specifications

Immunogen

This NfkB p65 antibody was raised against a synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein.

Reactivity Notes

Predicted to react with Bovine, Gorilla, New World Monkey and Porcine.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free

Western Blot: RelA/NFkB p65 Antibody (112A1021)Azide and BSA Free [NBP2-27416]

Western Blot: RelA/NFkB p65 Antibody (112A1021)Azide and BSA Free [NBP2-27416]

Western Blot: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 ug/mL mouse anti-RelA/NFkB p65 monoclonal (NB100-56712, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG (HAF018). Image using the standard format of this product.
Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free [NBP2-27416]

Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free [NBP2-27416]

Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Formalin-fixed, paraffin-embedded ovarian cystadenocarcinoma probed with p65 antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Flow Cytometry: RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free [NBP2-27416]

Flow Cytometry: RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free [NBP2-27416]

Flow Cytometry: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Intracellular staining of 293 HEK cells using 0.5 ug of p65 antibody. Green histogram represents the isotype control (p65) antibody. this antibody was used for this test, and an anti-mouse IgG PE conjugated secondary antibody.
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Applications for RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free

Application
Recommended Usage

Flow (Intracellular)

reported in scientific literature (PMID 15955098)

Flow Cytometry

1ul/1 million cells

Immunohistochemistry-Paraffin

5ug/ml. Use reported in scientific literature (Chang et al (2005))

Western Blot

2-5ug/ml~. Use reported in scientific literature (Wittmann et al (2005))
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 ml sterile PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RelA/NFkB p65

NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog A

Alternate Names

NFkB p65, NFKB3, p65RelA

Entrez Gene IDs

5970 (Human); 18033 (Mouse); 81736 (Rat)

Gene Symbol

RELA

Additional RelA/NFkB p65 Products

Product Documents for RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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