Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Flow Cytometry Control, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 112A1021

Format

BSA Free

Concentration

1.0 mg/ml

View all RelA/NFkB p65 Antibodies »

Product Specifications

Immunogen

This NfkB p65 antibody was raised against a synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein.

Reactivity Notes

Predicted to react with Bovine, Gorilla, New World Monkey and Porcine.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for RelA/NFkB p65 Antibody (112A1021) - BSA Free

Western Blot: RelA/NFkB p65 Antibody (112A1021)BSA Free [NB100-56712]

Western Blot: RelA/NFkB p65 Antibody (112A1021)BSA Free [NB100-56712]

Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Analysis of P65 using p65 antibody at 2 ug/mL in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.
Simple Western: RelA/NFkB p65 Antibody (112A1021)BSA Free [NB100-56712]

Simple Western: RelA/NFkB p65 Antibody (112A1021)BSA Free [NB100-56712]

Simple Western: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Lane view shows a specific band for NFkB p65 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: RelA/NFkB p65 Antibody (112A1021)BSA Free [NB100-56712]

Western Blot: RelA/NFkB p65 Antibody (112A1021)BSA Free [NB100-56712]

Western Blot: RelA/NFkB p65 Antibody (112A1021) [NB100-56712] - Lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 ug/mL mouse anti-RelA/NFkB p65 monoclonal (NB100-56712, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG (HAF018).
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Applications for RelA/NFkB p65 Antibody (112A1021) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

reported in scientific literature (Wittmann et al (2005))

Flow Cytometry

0.25 - 0.5 ug/10^6 cells

Immunohistochemistry

5 - 15 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL. Use reported in scientific literature (Chang et al (2005))

Immunoprecipitation

reported by customer review

Simple Western

1:25. Use reported by customer review

Western Blot

2 - 5 ug/mL. Use reported in scientific literature (Wittmann et al (2005))
Application Notes
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-56712 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RelA/NFkB p65

NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog A

Alternate Names

NFkB p65, NFKB3, p65RelA, anti-NF kB p65, anti-NFkB p65, anti-NF-kB p65, anti-p65, anti-RelA, anti-rela p65, p65 flow cytometry, p65 immunohistochemistry

Entrez Gene IDs

5970 (Human); 18033 (Mouse); 81736 (Rat)

Gene Symbol

RELA

Additional RelA/NFkB p65 Products

Product Documents for RelA/NFkB p65 Antibody (112A1021) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RelA/NFkB p65 Antibody (112A1021) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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