RelA/NFkB p65 Antibody Pack
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20216
Key Product Details
Species
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Product Summary for RelA/NFkB p65 Antibody Pack
This pack contains 1 vial each of: MAB7226-SP (25 ug), MAB7226-SP (0.025 mg), NB100-56712SS (25 ug), MAB7624-SP (1 mL).
Product Specifications
Application Notes
See individual datasheets of components for their validated applications
Reactivity Notes
See individual datasheets of components for their validated species
Scientific Data Images for RelA/NFkB p65 Antibody Pack
Detection of Phospho-RelA/NF kappa B p65 (S536) in Treated HT-29 and Treated HeLa Cell Lines by Western Blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Phospho-RelA/NF kappa B p65 (S536) Monoclonal Antibody (Catalog # MAB7226) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-RelA/ NF kappa B p65 (S536) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of RelA/NFkB p65 in Ramos, Daudi and NIH-3T3 Cell Lines by Western Blot.
Analysis of P65 using p65 antibody at 2 ug/mL in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.Western blot shows 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell line lysates. RelA/NFkB p65 was detected with 2 µg/mL of Mouse Anti-Human RelA/NFkB p65 (112A1021) Monoclonal Antibody (Catalog # NB100-56712) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. This experiment was conducted under reducing conditions.
Detection of Phospho-RelA/NF kappa B p65 (S529) in Treated HeLa by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-RelA/NF kappa B p65 (S529) Monoclonal Antibody (Catalog # MAB7624) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-RelA/NF kappa B p65 (S529) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Kit Contents for RelA/NFkB p65 Antibody Pack
- MAB7226-SP
- MAB7624-SP
- HAF007: Mouse IgG Horseradish Peroxidase-conjugated Antibody
- NB100-56712SS
Formulation, Preparation, and Storage
Concentration
Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.
Storage
Storage is content dependent.
Background: RelA/NFkB p65
Long Name
v-rel Reticuloendotheliosis Viral Oncogene Homolog A
Alternate Names
NFkB p65, NFKB3, p65RelA
Gene Symbol
RELA
Additional RelA/NFkB p65 Products
Product Documents for RelA/NFkB p65 Antibody Pack
Product Specific Notices for RelA/NFkB p65 Antibody Pack
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.
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