RelA/NFkB p65 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56502

Novus Biologicals, part of Bio-Techne
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NB100-56502
NB100-56502SS
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human

Predicted:

Canine (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all RelA/NFkB p65 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids within 1 and 50 of human p65 protein was used as immunogen.

Reactivity Notes

Reacts with Chimpanzee and New World Monkey. Predicted to react with Canine, Mouse and Rat.

Specificity

The amino acid sequence used as immunogen is 100% homologous in human (isoform CRA_a, CRA_c)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RelA/NFkB p65 Antibody

Western Blot: RelA/NFkB p65 Antibody [NB100-56502]

Western Blot: RelA/NFkB p65 Antibody [NB100-56502]

Western Blot: RelA/NFkB p65 Antibody [NB100-56502] - Analysis of extracts from various cell lines using NFkB p65 antibody at 1:100 (5 ug/mL).
Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 Antibody [NB100-56502]

Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 Antibody [NB100-56502]

Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody [NB100-56502] - p65 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: RelA/NFkB p65 Antibody [NB100-56502]

Western Blot: RelA/NFkB p65 Antibody [NB100-56502]

Western Blot: RelA/NFkB p65 Antibody [NB100-56502] - Analysis of p65 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody.

Applications for RelA/NFkB p65 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Western Blot

1:100 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 0.2% gelatin

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RelA/NFkB p65

NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog A

Alternate Names

NFkB p65, NFKB3, p65RelA

Entrez Gene IDs

5970 (Human)

Gene Symbol

RELA

UniProt

Q04206

Additional RelA/NFkB p65 Products

Product Documents for RelA/NFkB p65 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RelA/NFkB p65 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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