RelA/NFkB p65 [p Ser276] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77827

Novus Biologicals, part of Bio-Techne
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NBP1-77827
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RelA/NFkB p65 Antibodies »

Product Specifications

Immunogen

RelA/NFkB p65 peptide corresponding to an internal region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). (Uniprot: Q04206)

Reactivity Notes

This phospho specific polyclonal antibody is specific for phosphorylated pS276 human RelA/NFkB p65. Reactivity with non-phosphorylated pS267 is minimal. Cross reactivity with pS276 phosphorylated RelA/NFkB p65 from mouse, rat or other species has not been determined.

Modification

p Ser276

Specificity

The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS276 human RelA/NFkB p65. Reactivity with non-phosphorylated pS267 is minimal. Cross reactivity with pS276 phosphorylated RelA/NFkB p65 from mouse, rat or other species has not been determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody RelA/NFkB p65 p Ser276 Antibody was prepared from monospecific antiserum by delipidation and defibrination

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for RelA/NFkB p65 [p Ser276] Antibody - BSA Free

Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77827]

Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77827]

Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77827] - Probed against Normal (Lane 1) and TNFalpha (Lane 2) Stimulated HeLa whole cell lysates. A band was observed between 55 and 72 kD corresponding to the expected MW of NFkB p65. The observed higher MW bands have not been characterized.

Applications for RelA/NFkB p65 [p Ser276] Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:25000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:1000
Application Notes
This product reacts with human pS276 p65 and shows minimal reactivity when tested by western blot with non-phosphorylated p65 and minimal reactivity when tested by ELISA against the non-phosphorylated form of the immunizing peptide. For immunoblotting a 1:1,000 dilution is recommended. A 65 kDa band corresponding to human p65 is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: RelA/NFkB p65

NF'B was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NF'B bound to I'B. NF'B was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), cRel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NF'B subunit p65, similar to p50/p65 heterodimers. Low levels of p52 and p50 homodimers can also exist in cells. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by I'B-a. I'B-a 'binds to the p65 subunit preventing nuclear localization and DNA binding. Activators mediate a rapid phosphorylation of I'B by I'B kinase (IKK) which results in subsequent ubiquitination and proteolytic degradation. NF'B is then transported to the nucleus, where it activates transcription of target genes through binding to NF'B target sequences within the promoter. The HTLV-I protein Tax can induce constitutive NF'B activation through phosphorylation of both I'B-a and I'B-B. The transforming protein Tax inhibits p53 transcriptional activity through the NF'B signaling pathway, specifically via the p65 (RelA) subunit. The inhibition of p53 activity is dependent upon phosphorylation of p65 (RelA) at S536 by the upstream kinase IKKB.

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog A

Alternate Names

NFkB p65, NFKB3, p65RelA, NF kB p65 phospho, NFkB p65 phospho, NF-kB p65 phospho, phospho NF kB p65, phospho NFkB p65, phospho NF-kB p65

Gene Symbol

RELA

UniProt

Q04206, Q99496 (Human)

Additional RelA/NFkB p65 Products

Product Documents for RelA/NFkB p65 [p Ser276] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RelA/NFkB p65 [p Ser276] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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