RelA/NFkB p65 NLS Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24541

Novus Biologicals, part of Bio-Techne
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NBP2-24541
NBP2-24541SS
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Bovine, Equine, Primate

Cited:

Human, Mouse

Predicted:

Canine (100%), Chicken (100%), Chimpanzee (100%), Drosophila (100%), Monkey (100%), Rat (100%), Rhesus Macaque (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all RelA/NFkB p65 NLS Antibodies »

Product Specifications

Immunogen

A portion of the NFkB p65 NLS nuclear localization signal (NLS) (amino acids DTDDRHRIEEKRKRKT) was used as the immunogen for this antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RelA/NFkB p65 NLS Antibody - BSA Free

Western Blot: RelA/NFkB p65 NLS AntibodyBSA Free [NBP2-24541]

Western Blot: RelA/NFkB p65 NLS AntibodyBSA Free [NBP2-24541]

Western Blot: RelA/NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody analysis of p65 NLS in Jurkat lysate using p65 NLS antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]

Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]

Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 NLS Antibody [NBP2-24541] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-RelA/NFkB p65 NLS Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]

Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody - BSA Free [NBP2-24541]

Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody [NBP2-24541] - NFkB p65 NLS Antibody Formalin-fixed, paraffin-embedded human liver stained with p65 NLS antibody at 10 ug/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
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Applications for RelA/NFkB p65 NLS Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1-3 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RelA/NFkB p65 NLS

Alternate Names

MGC131774, NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65)), Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65, p65 NLS, p65 Nuclear Localization Sequence, p65-NLS, v-rel reticuloendotheliosis viral oncogene homolog A (avian), v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Gene Symbol

RELA

Additional RelA/NFkB p65 NLS Products

Product Documents for RelA/NFkB p65 NLS Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RelA/NFkB p65 NLS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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