Respiratory Syncytial Virus Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30413

Novus Biologicals, part of Bio-Techne
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NBP1-30413
NBP1-30413SS
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Key Product Details

Species Reactivity

Virus

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Respiratory Syncytial Virus Antibodies »

Product Specifications

Immunogen

Whole RSV virions (subgroup A-Long strain) were used as an immunogen.

Reactivity Notes

Virus

Specificity

Whole RSV virions (subgroup A-Long strain) were used as an immunogen. Detects G, F, N and M2 proteins of RSV.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Respiratory Syncytial Virus Antibody

Western Blot: Respiratory Syncytial Virus Antibody [NBP1-30413]

Western Blot: Respiratory Syncytial Virus Antibody [NBP1-30413]

Western Blot: Respiratory Syncytial Virus Antibody [NBP1-30413] - For WB analysis of Respiratory Syncytial Virus (RSV), 3 ug of RSV virions (subgroup A-Long strain) were loaded on SDS-PAGE, transferred to a PVDF membrane and detected with NBP1-30413 at a dilution of 1:1000 . Goat anti-rabbit secondary antibody was used at 1:10,000 dilution. The antibody detects the following RSV proteins: Glycoprotein (G) ~90 kDa, Fusion (F) protein) ~55 kDa, and nucleocapsid (N) protein) ~46 kDa. Overexposure of the blot can result in detection of M2 protein (~22 kDA-not shown).

Applications for Respiratory Syncytial Virus Antibody

Application
Recommended Usage

Western Blot

1:500 -1:1000
Application Notes
This antibody is useful in Western blot.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Respiratory Syncytial Virus

RSV is a negative sense RNA paramyxovirus in which the major virus neutralization antigens are the glycoprotein (G) and the fusion (F) protein that mediate attachment and fusion to the host cell during virus infection, respectively. Other RSV proteins include the nucleocapsid protein (N) and the M2 protein. The two major subgroups of RSV are A and B with the A strain being more virulent and responsible for the majority of severe RSV infections. Respiratory syncytial virus (RSV) infection is responsible for a large proportion of viral respiratory infections, and significant morbidity is associated with RSV infections. To date, few RSV vaccine candidates have shown promise with no human vaccine yet been proven safe and efficacious.

Alternate Names

Fusion glycoprotein F0, Fusion glycoprotein F1, Fusion glycoprotein F2, RS virus, RSV

Additional Respiratory Syncytial Virus Products

Product Documents for Respiratory Syncytial Virus Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Respiratory Syncytial Virus Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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