Retinol Binding Protein RBP Antibody (OTI2H3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73852

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2H3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Retinol Binding Protein RBP Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RBP1 (NP_002890) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

15.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Retinol Binding Protein RBP Antibody (OTI2H3) - Azide and BSA Free

Western Blot: Retinol Binding Protein RBP Antibody (OTI2H3)Azide and BSA Free [NBP2-73852]

Western Blot: Retinol Binding Protein RBP Antibody (OTI2H3)Azide and BSA Free [NBP2-73852]

Western Blot: Retinol Binding Protein RBP Antibody (OTI2H3) - Azide and BSA Free [NBP2-73852] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Retinol Binding Protein RBP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with
Immunocytochemistry/ Immunofluorescence: Retinol Binding Protein RBP Antibody (OTI2H3) - Azide and BSA Free [NBP2-73852]

Immunocytochemistry/ Immunofluorescence: Retinol Binding Protein RBP Antibody (OTI2H3) - Azide and BSA Free [NBP2-73852]

Immunocytochemistry/Immunofluorescence: Retinol Binding Protein RBP Antibody (OTI2H3) - Azide and BSA Free [NBP2-73852] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Retinol Binding Protein RBP.
Immunohistochemistry: Retinol Binding Protein RBP Antibody (OTI2H3) - Azide and BSA Free [NBP2-73852]

Immunohistochemistry: Retinol Binding Protein RBP Antibody (OTI2H3) - Azide and BSA Free [NBP2-73852]

Immunohistochemistry: Retinol Binding Protein RBP Antibody (OTI2H3) - Azide and BSA Free [NBP2-73852] - Staining of paraffin-embedded Human liver tissue using anti-Retinol Binding Protein RBP mouse monoclonal antibody.
View 6 more images

Applications for Retinol Binding Protein RBP Antibody (OTI2H3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Retinol Binding Protein RBP

RBP1 is the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. The gene harbors four exons encoding 24, 59, 33, and 16 amino acid residues respectively. The second intervening sequence alone occupies 19 kb of the 21 kb of the gene.

Alternate Names

C, Cellular retinol-binding protein, CRABP-I, CRBP1CRBP-I, CRBPCellular retinol-binding protein I, CRBPI, RBPC, retinol binding protein 1, cellular, retinol-binding protein 1, retinol-binding protein 1, cellular

Gene Symbol

RBP1

Additional Retinol Binding Protein RBP Products

Product Documents for Retinol Binding Protein RBP Antibody (OTI2H3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Retinol Binding Protein RBP Antibody (OTI2H3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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