Retinol Binding Protein RBP Antibody (SPM442)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34362
![Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (SPM442) [NBP2-34362]](https://resources.bio-techne.com/images/products/Retinol-Binding-Protein-RBP-Antibody-SPM442-Immunohistochemistry-Paraffin-NBP2-34362-img0001.jpg "Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (SPM442) [NBP2-34362]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Goat, Monkey, Rabbit
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM442
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Retinol binding protein purified from human plasma
Epitope
aa 74-182
Localization
Cytoplasmic
Specificity
Recognizes a protein of 21kDa-25kDa, identified as retinol binding protein (RBP). Its epitope localizes between aa 74-182 of human RBP. This monoclonal antibody recognizes reduced and carboxy-methylated RBP (RCM-RBP) as well as the circulatory RBP but not the native RBP, thereby suggesting that its epitope becomes accessible either on unfolding or upon binding of RBP to transthyretin (prealbumin). RBP is responsible for distributing retinol from the retinoid stores in the liver to the various target tissues. Once secreted into the blood with bound retinol, the vitamin carrier circulates complexed with transthyretin prior to vitamin delivery at the plasma membrane through a receptor-mediated mechanism.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34410)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Retinol Binding Protein RBP Antibody (SPM442)
Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (SPM442) [NBP2-34362]
Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody (SPM442) [NBP2-34362] - Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with Retinol Binding Protein RBP antibody (SPM442).Applications for Retinol Binding Protein RBP Antibody (SPM442)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Retinol Binding Protein RBP
Alternate Names
C, Cellular retinol-binding protein, CRABP-I, CRBP1CRBP-I, CRBPCellular retinol-binding protein I, CRBPI, RBPC, retinol binding protein 1, cellular, retinol-binding protein 1, retinol-binding protein 1, cellular
Gene Symbol
RBP1
UniProt
Additional Retinol Binding Protein RBP Products
Product Documents for Retinol Binding Protein RBP Antibody (SPM442)
Product Specific Notices for Retinol Binding Protein RBP Antibody (SPM442)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...