Retinol Binding Protein RBP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1494
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence VEGVVCKQVFKKVQ corresponding to C-Terminus according to NP_002890.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Retinol Binding Protein RBP Antibody
Western Blot: Retinol Binding Protein RBP Antibody [NB100-1494]
Western Blot: Retinol Binding Protein RBP Antibody [NB100-1494] - Staining of NIH3T3 embryonic mouse cell lysate with antibody at 2 ug/mL (A) and U251 cell lysate with antibody at 0.5 ug/mL (B). 35 ug protein each in RIPA buffer.Immunocytochemistry/ Immunofluorescence: Retinol Binding Protein RBP Antibody [NB100-1494]
Immunocytochemistry/Immunofluorescence: Retinol Binding Protein RBP Antibody [NB100-1494] - Analysis of paraformaldehyde fixed U-2 OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Flow Cytometry: Retinol Binding Protein RBP Antibody [NB100-1494]
Flow Cytometry: Retinol Binding Protein RBP Antibody [NB100-1494] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for Retinol Binding Protein RBP Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:4000
Western Blot
0.5 - 3 ug/mL
Application Notes
IHC-P has been reported for previous lots.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Retinol Binding Protein RBP
Alternate Names
C, Cellular retinol-binding protein, CRABP-I, CRBP1CRBP-I, CRBPCellular retinol-binding protein I, CRBPI, RBPC, retinol binding protein 1, cellular, retinol-binding protein 1, retinol-binding protein 1, cellular
Gene Symbol
RBP1
UniProt
Additional Retinol Binding Protein RBP Products
Product Documents for Retinol Binding Protein RBP Antibody
Product Specific Notices for Retinol Binding Protein RBP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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