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Retinol Binding Protein RBP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20132

Novus Biologicals, part of Bio-Techne
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NBP2-20132

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Retinol Binding Protein RBP. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Retinol Binding Protein RBP Antibody

Western Blot: Retinol Binding Protein RBP Antibody [NBP2-20132]

Western Blot: Retinol Binding Protein RBP Antibody [NBP2-20132]

Western Blot: Retinol Binding Protein RBP Antibody [NBP2-20132] - Elevation of retinal tissue induction of visual cycle enzymes stimulated by retinoic acid 6 (STRA6, C) in chrysin-treated mice. The db/db mice were orally supplemented with 10 mg/kg of chrysin daily for 10 weeks. The db/m mice were introduced as control animals. Mouse retinal tissue extracts were subject to Western blot analysis with a primary antibody against each target protein of LRAT, RDH5, cellular retinol binding protein (CRBP), CRALBP, IRBP, or STRA6. beta-actin protein was used as an internal control. Bar graphs (mean +- SEM, n = nine independent experiments) in the bottom or right panels represent densitometric results of upper blot bands. * Values in bar graphs indicate a significant difference at p<0.05. Image collected and cropped by CiteAb from the following publication (mdpi.com/2072-6643/10/8/1046), licensed under a CC-BY license
Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody [NBP2-20132]

Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody [NBP2-20132]

Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody [NBP2-20132] - Human colon, at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Retinol Binding Protein RBP Antibody

Immunocytochemistry/ Immunofluorescence: Retinol Binding Protein RBP Antibody [NBP2-20132] -

Immunocytochemistry/ Immunofluorescence: Retinol Binding Protein RBP Antibody [NBP2-20132] - Retinol Binding Protein RBP antibody detects Retinol Binding Protein RBP protein at cytoplasm by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Retinol Binding Protein RBP stained by Retinol Binding Protein RBP antibody (NBP2-20132) diluted at 1:500.
Blue: Fluoroshield with DAPI .
Scale bar= 10 um.

Applications for Retinol Binding Protein RBP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Retinol Binding Protein RBP

This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

C, Cellular retinol-binding protein, CRABP-I, CRBP1CRBP-I, CRBPCellular retinol-binding protein I, CRBPI, RBPC, retinol binding protein 1, cellular, retinol-binding protein 1, retinol-binding protein 1, cellular

Gene Symbol

RBP1

UniProt

Additional Retinol Binding Protein RBP Products

Product Documents for Retinol Binding Protein RBP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Retinol Binding Protein RBP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.