RFC2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32253

Novus Biologicals, part of Bio-Techne
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NBP1-32253
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (95%), Chicken (93%), Mouse (96%), Rat (96%), Rhesus Macaque (99%), Xenopus (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RFC2 Antibodies »

Product Summary for RFC2 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human RFC2. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (91%), Zebrafish (87%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RFC2 Antibody

Western Blot: RFC2 Antibody [NBP1-32253]

Western Blot: RFC2 Antibody [NBP1-32253]

Western Blot: RFC2 Antibody [NBP1-32253] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC2 antibody diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: RFC2 Antibody [NBP1-32253]

Immunocytochemistry/ Immunofluorescence: RFC2 Antibody [NBP1-32253]

Immunocytochemistry/Immunofluorescence: RFC2 Antibody [NBP1-32253] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Western Blot: RFC2 Antibody [NBP1-32253]

Western Blot: RFC2 Antibody [NBP1-32253]

Western Blot: RFC2 Antibody [NBP1-32253] - A: A431 B: H1299 C: Hela 10% SDS PAGE; antibody diluted at 1:1000

Applications for RFC2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RFC2

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Alternate Names

A1, A1 40 kDa subunit, Activator 1 40 kDa subunit, Activator 1 subunit 2, MGC3665, replication factor C (activator 1) 2, 40kDa, replication factor C 2, Replication factor C 40 kDa subunit, replication factor C subunit 2, RF-C 40 kDa subunit, RFC40replication factor C (activator 1) 2 (40kD)

Gene Symbol

RFC2

Additional RFC2 Products

Product Documents for RFC2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RFC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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