RFC2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89341
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PAPAFSKAPGSAGHYELPWVEKYRPVKLNEIVGNEDTVSRLEVFAREGNVPNIIIAGPPGTGKTTSILCLARALLGPALKDAMLELNASND
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RFC2 Antibody
Western Blot: RFC2 Antibody [NBP1-89341]
Western Blot: RFC2 Antibody [NBP1-89341] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).Immunocytochemistry/ Immunofluorescence: RFC2 Antibody [NBP1-89341]
Immunocytochemistry/Immunofluorescence: RFC2 Antibody [NBP1-89341] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.Immunohistochemistry-Paraffin: RFC2 Antibody [NBP1-89341]
Immunohistochemistry-Paraffin: RFC2 Antibody [NBP1-89341] - Staining of human vulva/anal skin shows strong nuclear positivity in squamous epithelial cells.Applications for RFC2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RFC2
Alternate Names
A1, A1 40 kDa subunit, Activator 1 40 kDa subunit, Activator 1 subunit 2, MGC3665, replication factor C (activator 1) 2, 40kDa, replication factor C 2, Replication factor C 40 kDa subunit, replication factor C subunit 2, RF-C 40 kDa subunit, RFC40replication factor C (activator 1) 2 (40kD)
Gene Symbol
RFC2
Additional RFC2 Products
Product Documents for RFC2 Antibody
Product Specific Notices for RFC2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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