Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3F7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all RFX3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RFX3 (NP_602304) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

83.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RFX3 Antibody (OTI3F7) - Azide and BSA Free

Western Blot: RFX3 Antibody (OTI3F7)Azide and BSA Free [NBP2-73865]

Western Blot: RFX3 Antibody (OTI3F7)Azide and BSA Free [NBP2-73865]

Western Blot: RFX3 Antibody (OTI3F7) - Azide and BSA Free [NBP2-73865] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFX3.

Applications for RFX3 Antibody (OTI3F7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RFX3

RFX3 is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Two transcript variants encoding different isoforms have been described for this gene, and at least one of the variants utilizes alternative polyadenylation signals. [provided by RefSeq]

Alternate Names

bA32F11.1, DNA binding protein RFX3, MGC87155, Regulatory factor X 3, regulatory factor X, 3 (influences HLA class II expression), transcription factor RFX3

Gene Symbol

RFX3

Additional RFX3 Products

Product Documents for RFX3 Antibody (OTI3F7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RFX3 Antibody (OTI3F7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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