RG9MTD1 Antibody

Immunoprecipitation: RG9MTD1 Antibody [NBP1-83654] -

Immunoprecipitation: RG9MTD1 Antibody [NBP1-83654] - P32 can be co-immunoprecipitated with mitochondrial RNase P protein.(A) RT-PCR assay for the mRNA levels of P32 or MRPP1 in siRNA treated HeLa cells. The error bars represent standard deviation of three replicates. (B) Northern hybridization for mitochondrial pre-rRNA. Total RNA prepared from HeLa cells pre-treated for 24 hrs with P32 [(−)P32] or MRPP1 [(−)MRPP1] siRNAs was analyzed by northern hybridization, using probes specific to pre-rRNA, as in Figure 5.The same blot was re-probed for 7 SL RNA, which served as a loading control. (C) Immunoprecipitation using MRPP1 antibody (MRPP1-AB) or RPL5 antibody (RPL5-AB) was performed as described in materials & methods. Co-isolated proteins were separated by SDS-PAGE, & P32 protein was determined by western analysis. (−)AB, control immunoprecipitation in the absence of antibody. (D) DNase I treatment disrupted the P32-MRPP1 interaction. Immunoprecipitation was performed using MRPP1 antibody, as in panel C. After wash, the beads were incubated with either RNase A or DNase I, to elute bound materials. The eluted proteins were analyzed by western blots for the presence of P32 protein. Buffer, 1×TE buffer alone. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23990920), licensed under a CC-BY license. Not internally tested by Novus Biologicals.