RGL2 Antibody (4D10)

ELISA: RGL2 Antibody (4D10) [H00005863-M02]

ELISA: RGL2 Antibody (4D10) [H00005863-M02] - Detection limit for recombinant GST tagged RGL2 is approximately 1ng/ml as a capture antibody.

Immunocytochemistry/ Immunofluorescence: RGL2 Antibody (4D10) [H00005863-M02] -

Immunocytochemistry/ Immunofluorescence: RGL2 Antibody (4D10) [H00005863-M02] - Localization of RGL2 at different endomembrane compartments. HEK-HT (Normal cells) & HEK-HT-H-RasV12 (Transformed cells) cells were fixed & imaged for endogenous RGL2 (IF anti-RGL2, represented in red), together with EEA1 (early endosome marker, IF anti-EEA1, represented in green), GFP-Rab11 (recycling endosome marker, represented in green), GFP-Rab6 (Trans-Golgi marker, represented in green), or iRFP-LC3 (autophagosome marker, represented in green). Representative confocal cross sections of normal cells are shown (left). Quantifications are reported for both normal & transformed cells (right). Localization was calculated as percentage (%) of the indicated endomembrane compartments positive for RGL2, as compared to the control pseudo-compartments. Each dot corresponds to one cell. Graph represents mean ± SEM of 21 to 40 cells from 3–4 independent experiments. For statistics Mann Whitney test was used. **p value < 0.01, ***p value < 0.001. Scale bars are 10 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31222145), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: RGL2 Antibody (4D10) [H00005863-M02] -

Western Blot: RGL2 Antibody (4D10) [H00005863-M02] - Impact of RGL2 depletion on RalB activation at autophagosomes upon starvation. (A) Modulation by autophagy of RalB activity at autophagosomes upon RGL2 depletion. Normal HEK-HT cells were transfected with indicated siRNAs (day 0), then transfected with FRET RalB biosensor & Cherry-LC3 plasmids (day 2), & incubated in either basal (DMEM) or starvation (EBSS) medium (4 hours) before imaging (day 3). Graph represents mean ± SEM of n = 17 to 57 cells per condition, from 3 independent experiments. To compare DMEM versus EBSS conditions Mann Whitney test was used, **p < 0.01, ***, & p < 0.001. (B) Validation of RGL2 depletions. Representative western blots for RGL2 & GAPDH from cell lysates of HEK-HT cells prepared 72 hrs after transfection with the indicated siRNAs. Quantifications of RGL2 protein depletion, normalized for siControl condition (=100), are shown below the WB. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31222145), licensed under a CC-BY license. Not internally tested by Novus Biologicals.