Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI10F2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all RGL2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 547-777 of human RGL2(NP_004752) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

83.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RGL2 Antibody (OTI10F2) - Azide and BSA Free

Western Blot: RGL2 Antibody (OTI10F2)Azide and BSA Free [NBP2-73875]

Western Blot: RGL2 Antibody (OTI10F2)Azide and BSA Free [NBP2-73875]

Western Blot: RGL2 Antibody (OTI10F2) - Azide and BSA Free [NBP2-73875] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGL2.
Immunohistochemistry: RGL2 Antibody (OTI10F2) - Azide and BSA Free [NBP2-73875]

Immunohistochemistry: RGL2 Antibody (OTI10F2) - Azide and BSA Free [NBP2-73875]

Immunohistochemistry: RGL2 Antibody (OTI10F2) - Azide and BSA Free [NBP2-73875] - Staining of paraffin-embedded Human Kidney tissue using anti-RGL2 mouse monoclonal antibody.
Immunohistochemistry: RGL2 Antibody (OTI10F2) - Azide and BSA Free [NBP2-73875]

Immunohistochemistry: RGL2 Antibody (OTI10F2) - Azide and BSA Free [NBP2-73875]

Immunohistochemistry: RGL2 Antibody (OTI10F2) - Azide and BSA Free [NBP2-73875] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RGL2 mouse monoclonal antibody.
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Applications for RGL2 Antibody (OTI10F2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RGL2

Probable guanine nucleotide exchange factor. Putative effector of Ras and/or Rap. Associates with the GTP-bound form of Rap 1A and H-Ras in vitro

Alternate Names

GDS-related protein, HKE1.5, KE1.5, RAB2, member RAS oncogene family-like, RAB2LRas-associated protein RAB2L, ral guanine nucleotide dissociation stimulator-like 2, RalGDS-like 2, RalGDS-like factor

Gene Symbol

RGL2

Additional RGL2 Products

Product Documents for RGL2 Antibody (OTI10F2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RGL2 Antibody (OTI10F2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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