RGS20 Antibody (3E10)
Novus Biologicals, part of Bio-Techne | Catalog # H00008601-M04
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3E10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RGS20 (NP_003693, 78 a.a. ~ 176 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NQEDQRPTIASHELRADLPTWEESPAPTLEEVNAWAQSFDKLMVTPAGRNAFREFLRTEFSEENMLFWMACEELKKEANKNIIEEKARIIYEDYISILS
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
RGS20 - regulator of G-protein signalling 20 (3E10)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for RGS20 Antibody (3E10)
![Western Blot: RGS20 Antibody (3E10) [H00008601-M04]](https://resources.bio-techne.com/images/products/RGS20-Antibody-3E10-Western-Blot-H00008601-M04-img0005.jpg "Western Blot: RGS20 Antibody (3E10) [H00008601-M04]")
Western Blot: RGS20 Antibody (3E10) [H00008601-M04]
Western Blot: RGS20 Antibody (3E10) [H00008601-M04] - Analysis of RGS20 expression in NIH/3T3 (Cat # L018V1).![Immunocytochemistry/ Immunofluorescence: RGS20 Antibody (3E10) [H00008601-M04]](https://resources.bio-techne.com/images/products/RGS20-Antibody-3E10-Immunocytochemistry-Immunofluorescence-H00008601-M04-img0003.jpg "Immunocytochemistry/ Immunofluorescence: RGS20 Antibody (3E10) [H00008601-M04]")
Immunocytochemistry/ Immunofluorescence: RGS20 Antibody (3E10) [H00008601-M04]
Immunocytochemistry/Immunofluorescence: RGS20 Antibody (3E10) [H00008601-M04] - Analysis of monoclonal antibody to RGS20 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: RGS20 Antibody (3E10) [H00008601-M04] - Detection limit for recombinant GST tagged RGS20 is 0.3 ng/ml as a capture antibody.
Applications for RGS20 Antibody (3E10)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RGS20
Alternate Names
G(z)GAP, Gz-GAP, Gz-selective GTPase-activating protein, regulator of G-protein signaling 20, Regulator of G-protein signaling Z1, regulator of G-protein signalling 20, Regulator of Gz-selective protein signaling 1, RGSZ1g(z)GAP, ZGAP1gz-GAP
Entrez Gene IDs
8601 (Human)
Gene Symbol
RGS20
UniProt
Additional RGS20 Products
Product Documents for RGS20 Antibody (3E10)
Product Specific Notices for RGS20 Antibody (3E10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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