RGS5 Antibody (OTI1C1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00880
![Immunohistochemistry-Paraffin: RGS5 Antibody (OTI1C1) [NBP2-00880]](https://resources.bio-techne.com/images/products/RGS5-Antibody-OTI1C1-Immunohistochemistry-Paraffin-NBP2-00880-img0002.jpg "Immunohistochemistry-Paraffin: RGS5 Antibody (OTI1C1) [NBP2-00880]")
![Immunohistochemistry: RGS5 Antibody (OTI1C1) [NBP2-00880]](https://resources.bio-techne.com/images/products/RGS5-Antibody-OTI1C1-Immunohistochemistry-NBP2-00880-img0005.jpg "Immunohistochemistry: RGS5 Antibody (OTI1C1) [NBP2-00880]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1C1
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human RGS5 (NP_003608) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
20.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RGS5 Antibody (OTI1C1)
Immunohistochemistry: RGS5 Antibody (OTI1C1) [NBP2-00880]
Immunohistochemistry: RGS5 Antibody (OTI1C1) [NBP2-00880] - Representative photomicrographs of hematoxylin and eosin and regulator of G-protein signaling (RGS) 5 in squamous cell carcinoma of the tongue in noninvasive and invasive portions. (a), (b), show H&E and RGS5 in noninvasive portions at 10x magnification. (f), (g), show H&E and RGS5 in noninvasive portions at 40x magnification. (k), (l) show H&E and RGS5 in invasive portions at 10x magnification. (p), (q), show H&E and RGS5 in invasive portions at 40x magnification. Image collected and cropped by CiteAb from the following publication (https://onlinelibrary.wiley.com/doi/abs/10.1002/cre2.166) licensed under a CC-BY license.![Western Blot: RGS5 Antibody (OTI1C1) [NBP2-00880]](https://resources.bio-techne.com/images/products/RGS5-Antibody-OTI1C1-Western-Blot-NBP2-00880-img0001.jpg "Western Blot: RGS5 Antibody (OTI1C1) [NBP2-00880]")
Western Blot: RGS5 Antibody (OTI1C1) [NBP2-00880]
Western Blot: RGS5 Antibody (OTI1C1) [NBP2-00880] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGS5.![Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880]](https://resources.bio-techne.com/images/products/RGS5-Antibody-OTI1C1-Flow-Cytometry-NBP2-00880-img0004.jpg "Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880]")
Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880]
Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880] - Flow cytometric Analysis of Jurkat cells, using anti-RGS5 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).![Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880]](https://resources.bio-techne.com/images/products/RGS5-Antibody-OTI1C1-Flow-Cytometry-NBP2-00880-img0003.jpg "Flow Cytometry: RGS5 Antibody (OTI1C1) [NBP2-00880]")
Applications for RGS5 Antibody (OTI1C1)
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RGS5
Alternate Names
MST092, MST106, MST129, MSTP032, MSTP092, MSTP106, MSTP129, regulator of G-protein signaling 5, regulator of G-protein signalling 5
Entrez Gene IDs
8490 (Human)
Gene Symbol
RGS5
Additional RGS5 Products
Product Documents for RGS5 Antibody (OTI1C1)
Product Specific Notices for RGS5 Antibody (OTI1C1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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