Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # Rheb 5D5

Format

BSA Free

Concentration

1 mg/ml

View all Rheb Antibodies »

Product Specifications

Immunogen

Human Rheb GST fusion

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Rheb Antibody (Rheb 5D5) - BSA Free

Application
Recommended Usage

Immunoprecipitation

5 ug/ml

Western Blot

1 ug/ml
Application Notes
Positive control(s): HeLa or 293T cell line.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rheb

Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.

Long Name

Ras Homolog Enriched In Brain

Alternate Names

Rheb2

Gene Symbol

RHEB

Additional Rheb Products

Product Documents for Rheb Antibody (Rheb 5D5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rheb Antibody (Rheb 5D5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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