RhoA Antibody (103)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90107
Recombinant Monoclonal Antibody
![Western Blot: RhoA Antibody (103) [NBP2-90107]](https://resources.bio-techne.com/images/products/RhoA-Antibody-103-Western-Blot-NBP2-90107-img0003.jpg "Western Blot: RhoA Antibody (103) [NBP2-90107]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 103
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human RhoA (Uniprot#: P61586-1; Met1-Leu193).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for RhoA Antibody (103)
Western Blot: RhoA Antibody (103) [NBP2-90107]
Western Blot: RhoA Antibody (103) [NBP2-90107] - Anti-RHOA rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDa. Observed band size:22 kDa.![Immunocytochemistry/ Immunofluorescence: RhoA Antibody (103) [NBP2-90107]](https://resources.bio-techne.com/images/products/RhoA-Antibody-103-Immunocytochemistry-Immunofluorescence-NBP2-90107-img0002.jpg "Immunocytochemistry/ Immunofluorescence: RhoA Antibody (103) [NBP2-90107]")
Immunocytochemistry/ Immunofluorescence: RhoA Antibody (103) [NBP2-90107]
Immunocytochemistry/Immunofluorescence: RhoA Antibody (103) [NBP2-90107] - Immunofluorescence staining of RHOA in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human RHOA monoclonal antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor-488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.Applications for RhoA Antibody (103)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RhoA
Alternate Names
h12, oncogene RHO H12, ras homolog gene family, member A, Rho cDNA clone 12, RHO12, RhoA, RHOH12ARH12ARHAAplysia ras-related homolog 12, small GTP binding protein RhoA, transforming protein RhoA
Gene Symbol
RHOA
Additional RhoA Products
Product Documents for RhoA Antibody (103)
Product Specific Notices for RhoA Antibody (103)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...