RhoA Antibody (1B12)

Novus Biologicals, part of Bio-Techne | Catalog # H00000387-M04

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Lambda Clone # 1B12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RhoA Antibodies »

Product Specifications

Immunogen

RHOA (AAH01360, 1 a.a. ~ 193 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL

Specificity

RHOA - ras homolog gene family, member A

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Lambda

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RhoA Antibody (1B12)

Western Blot: RhoA Antibody (1B12) [H00000387-M04]

Western Blot: RhoA Antibody (1B12) [H00000387-M04]

Western Blot: RhoA Antibody (1B12) [H00000387-M04] - Analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody (M04), clone 1B12. Lane 1: RHOA transfected lysatE (21.8 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: RhoA Antibody (1B12) [H00000387-M04]

Immunocytochemistry/ Immunofluorescence: RhoA Antibody (1B12) [H00000387-M04]

Immunocytochemistry/Immunofluorescence: RhoA Antibody (1B12) [H00000387-M04] - Analysis of monoclonal antibody to RHOA on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: RhoA Antibody (1B12) [H00000387-M04]

Immunohistochemistry-Paraffin: RhoA Antibody (1B12) [H00000387-M04]

Immunohistochemistry-Paraffin: RhoA Antibody (1B12) [H00000387-M04] - Analysis of monoclonal antibody to RHOA on formalin-fixed paraffin-embedded human lymphoma tissue.Antibody concentration 5 ug/ml
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Applications for RhoA Antibody (1B12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RhoA

RhoA belongs to the small GTPase superfamily, Rho family. It regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesion and actin stress fibers. It serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. It binds PRKCL1, ROCK1 and ROCK2. Interacts with NET1 and ARHGEF3.

Alternate Names

h12, oncogene RHO H12, ras homolog gene family, member A, Rho cDNA clone 12, RHO12, RhoA, RHOH12ARH12ARHAAplysia ras-related homolog 12, small GTP binding protein RhoA, transforming protein RhoA

Gene Symbol

RHOA

OMIM

165390 (Human)

UniProt

AAH01360 (Human)

Additional RhoA Products

Product Documents for RhoA Antibody (1B12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RhoA Antibody (1B12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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