RhoA Antibody (1C3)
Novus Biologicals, part of Bio-Techne | Catalog # H00000387-M08
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Lambda Clone # 1C3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RHOA (AAH01360, 1 a.a. ~ 193 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL
Specificity
RHOA - ras homolog gene family, member A
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 Lambda
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for RhoA Antibody (1C3)
![Western Blot: RhoA Antibody (1C3) [H00000387-M08]](https://resources.bio-techne.com/images/products/RhoA-Antibody-1C3-Western-Blot-H00000387-M08-img0005.jpg "Western Blot: RhoA Antibody (1C3) [H00000387-M08]")
Western Blot: RhoA Antibody (1C3) [H00000387-M08]
Western Blot: RhoA Antibody (1C3) [H00000387-M08] - Analysis of RHOA expression in A-431 (Cat # L015V1).![Immunocytochemistry/ Immunofluorescence: RhoA Antibody (1C3) [H00000387-M08]](https://resources.bio-techne.com/images/products/RhoA-Antibody-1C3-Immunocytochemistry-Immunofluorescence-H00000387-M08-img0003.jpg "Immunocytochemistry/ Immunofluorescence: RhoA Antibody (1C3) [H00000387-M08]")
Immunocytochemistry/ Immunofluorescence: RhoA Antibody (1C3) [H00000387-M08]
Immunocytochemistry/Immunofluorescence: RhoA Antibody (1C3) [H00000387-M08] - Analysis of monoclonal antibody to RHOA on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: RhoA Antibody (1C3) [H00000387-M08] - Detection limit for recombinant GST tagged RHOA is approximately 3ng/ml as a capture antibody.
Applications for RhoA Antibody (1C3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RhoA
Alternate Names
h12, oncogene RHO H12, ras homolog gene family, member A, Rho cDNA clone 12, RHO12, RhoA, RHOH12ARH12ARHAAplysia ras-related homolog 12, small GTP binding protein RhoA, transforming protein RhoA
Gene Symbol
RHOA
OMIM
165390 (Human)
UniProt
Additional RhoA Products
Product Documents for RhoA Antibody (1C3)
Product Specific Notices for RhoA Antibody (1C3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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