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RhoA Antibody (3G7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26344

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-26344-100ul
NBP3-26344-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3G7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from Human RHOA [UniProt P61586]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RhoA Antibody (3G7)

RhoA Antibody (3G7)

Immunohistochemistry: RhoA Antibody (3G7) [NBP3-26344] -

Immunohistochemistry: RhoA Antibody (3G7) [NBP3-26344] - Image of RhoA Antibody (3G7) diluted at 1:100 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for RhoA Antibody (3G7)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: RhoA

RhoA belongs to the small GTPase superfamily, Rho family. It regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesion and actin stress fibers. It serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. It binds PRKCL1, ROCK1 and ROCK2. Interacts with NET1 and ARHGEF3.

Alternate Names

h12, oncogene RHO H12, ras homolog gene family, member A, Rho cDNA clone 12, RHO12, RhoA, RHOH12ARH12ARHAAplysia ras-related homolog 12, small GTP binding protein RhoA, transforming protein RhoA

Gene Symbol

RHOA

Additional RhoA Products

Product Documents for RhoA Antibody (3G7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RhoA Antibody (3G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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