RhoA Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15485

Novus Biologicals, part of Bio-Techne
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NBP3-15485-20ul
NBP3-15485-100ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RhoA Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RhoA (NP_001655.1). MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RhoA Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RhoA Antibody - BSA Free [NBP3-15485]

Immunocytochemistry/ Immunofluorescence: RhoA Antibody - BSA Free [NBP3-15485]

Immunocytochemistry/Immunofluorescence: RhoA Antibody [NBP3-15485] - Immunofluorescence analysis of U2OS cells using RhoA antibody (NBP3-15485) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: RhoA Antibody - BSA Free [NBP3-15485]

Immunohistochemistry-Paraffin: RhoA Antibody - BSA Free [NBP3-15485]

Immunohistochemistry-Paraffin: RhoA Antibody [NBP3-15485] - Human colon carcinoma using [KO Validated] RhoA Rabbit pAb (NBP3-15485) at dilution of 1:350 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Knockout Validated: RhoA Antibody - BSA Free [NBP3-15485]

Knockout Validated: RhoA Antibody - BSA Free [NBP3-15485]

Knockout Validated: RhoA Antibody [NBP3-15485] - Western blot analysis of extracts from normal (control) and RhoA knockout (KO) HeLa cells, using RhoA antibody (NBP3-15485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for RhoA Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:100 - 1:500

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RhoA

RhoA belongs to the small GTPase superfamily, Rho family. It regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesion and actin stress fibers. It serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. It binds PRKCL1, ROCK1 and ROCK2. Interacts with NET1 and ARHGEF3.

Alternate Names

h12, oncogene RHO H12, ras homolog gene family, member A, Rho cDNA clone 12, RHO12, RhoA, RHOH12ARH12ARHAAplysia ras-related homolog 12, small GTP binding protein RhoA, transforming protein RhoA

Gene Symbol

RHOA

Additional RhoA Products

Product Documents for RhoA Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RhoA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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