Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Vertebrate

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1D4

Concentration

1 mg/ml

View all Rhodopsin Antibodies »

Product Specifications

Immunogen

Bovine Rhodopsin

Epitope

binds to the C- terminal epitope-T-E-T-S-Q-V-A-P-A- (COOH) of rhodopsin

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28672005).

Localization

Membrane

Specificity

Detects ~40kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Proteins containing the epitope tag can be selectively bound to a Rho 1D4 affinity matrix and eluted using an excess of -T-E-T-S-Q-V-A-P-A- peptide under mild conditions.

Scientific Data Images for Rhodopsin Antibody (1D4)

Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602]

Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602]

Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Quantification of rhodopsin protein (western blot) levels in the neural retina of WT (n = 7) and hFATP1TG (n = 8) mice. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28672005/) licensed under a CC-BY license.
Immunohistochemistry: Rhodopsin Antibody (1D4) [NBP1-47602]

Immunohistochemistry: Rhodopsin Antibody (1D4) [NBP1-47602]

Immunohistochemistry: Rhodopsin Antibody (1D4) [NBP1-47602] - Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 (NBP1-47602). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP1-47602) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.
Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602]

Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602]

Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Western Blot analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 (NBP1-47602). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP1-47602) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Applications for Rhodopsin Antibody (1D4)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:1000
Application Notes
1 ug/ml of Rhodopsin Antibody was sufficient for detection of rhodopsin in 10 ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rhodopsin

Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

Long Name

Rhodopsin

Alternate Names

OPN2, Opsin-2, RHO

Gene Symbol

RHO

UniProt

P08100 (Human)

Additional Rhodopsin Products

Product Documents for Rhodopsin Antibody (1D4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rhodopsin Antibody (1D4)

Proteins containing the epitope tag can be selectively bound to a Rho 1D4 affinity matrix and eluted using an excess of -T-E-T-S-Q-V-A-P-A- peptide under mild conditions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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