Rhodopsin Antibody (5N3G9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16655
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5N3G9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 249-348 of human Rhodopsin (NP_000530.1). EVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAIYNPVIYIMMNKQFRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Rhodopsin Antibody (5N3G9)
![Western Blot: Rhodopsin Antibody (5N3G9) [NBP3-16655]](https://resources.bio-techne.com/images/products/Rhodopsin-Antibody-5N3G9-Western-Blot-NBP3-16655-img0002.jpg "Western Blot: Rhodopsin Antibody (5N3G9) [NBP3-16655]")
Western Blot: Rhodopsin Antibody (5N3G9) [NBP3-16655]
Western Blot: Rhodopsin Antibody (5N3G9) [NBP3-16655] - Western blot analysis of extracts of various cell lines, using Rhodopsin Rabbit mAb (NBP3-16655) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.![Immunohistochemistry-Paraffin: Rhodopsin Antibody (5N3G9) [NBP3-16655]](https://resources.bio-techne.com/images/products/Rhodopsin-Antibody-5N3G9-Immunohistochemistry-Paraffin-NBP3-16655-img0001.jpg "Immunohistochemistry-Paraffin: Rhodopsin Antibody (5N3G9) [NBP3-16655]")
Immunohistochemistry-Paraffin: Rhodopsin Antibody (5N3G9) [NBP3-16655]
Immunohistochemistry-Paraffin: Rhodopsin Antibody (5N3G9) [NBP3-16655] - Immunohistochemistry of paraffin-embedded mouse retina using Rhodopsin Rabbit mAb (NBP3-16655) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for Rhodopsin Antibody (5N3G9)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Rhodopsin
Long Name
Rhodopsin
Alternate Names
OPN2, Opsin-2, RHO
Gene Symbol
RHO
Additional Rhodopsin Products
Product Documents for Rhodopsin Antibody (5N3G9)
Product Specific Notices for Rhodopsin Antibody (5N3G9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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