Rhodopsin Antibody (Rho 1D4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81059
Recombinant Monoclonal Antibody
![Western Blot: Rhodopsin Antibody (Rho 1D4)Azide and BSA Free [NBP2-81059]](https://resources.bio-techne.com/images/products/Rhodopsin-Antibody-Rho-1D4-Western-Blot-NBP2-81059-img0001.jpg "Western Blot: Rhodopsin Antibody (Rho 1D4)Azide and BSA Free [NBP2-81059]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Amphibian, Bovine, Zebrafish
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # Rho 1D4
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Bleached bovine rod outer segment (ROS).
Specificity
The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for Rhodopsin Antibody (Rho 1D4) - Azide and BSA Free
Western Blot: Rhodopsin Antibody (Rho 1D4)Azide and BSA Free [NBP2-81059]
Western Blot: Rhodopsin Antibody (Rho 1D4) [NBP2-81059] - Western Blot using anti-Rhodopsin antibody (Rho 1D4) [NBP2-81059]. Rat eye lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of Rho 1D4 [NBP2-81059] at 0.0001 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for monomeric rat ( Rattus norvegicus ) Rhodopsin is 39.0kDa, though this protein in known to form dimers and higher order multimers (c.f. Mansoor et al., PMID: 16492772, PMID: 10798675). NBP2-81059 successfully detected monomeric, dimeric and multimeric Rhodopsin in rat eye lysate.Applications for Rhodopsin Antibody (Rho 1D4) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Radioimmunoassay
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein-coupled receptors (GPCRs). The linear epitope (TETSQVAPA) can be used as a protein tag.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Rhodopsin
Long Name
Rhodopsin
Alternate Names
OPN2, Opsin-2, RHO
Gene Symbol
RHO
Additional Rhodopsin Products
Product Documents for Rhodopsin Antibody (Rho 1D4) - Azide and BSA Free
Product Specific Notices for Rhodopsin Antibody (Rho 1D4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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