Rhodopsin Antibody (Rho 1D4) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81059B
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Amphibian, Bovine, Zebrafish
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot
Label
Biotin
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # Rho 1D4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Bleached bovine rod outer segment (ROS).
Specificity
The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Applications for Rhodopsin Antibody (Rho 1D4) [Biotin]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Radioimmunoassay
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Rhodopsin
Long Name
Rhodopsin
Alternate Names
OPN2, Opsin-2, RHO
Gene Symbol
RHO
Additional Rhodopsin Products
Product Documents for Rhodopsin Antibody (Rho 1D4) [Biotin]
Product Specific Notices for Rhodopsin Antibody (Rho 1D4) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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