RhoGAP Antibody (SR1304)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22307

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-22307-100ul
NBP3-22307-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1304

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RhoGAP Antibodies »

Product Summary for RhoGAP Antibody (SR1304)

Immunogen

A synthesized peptide derived from human RhoGAP (Uniprot #: Q13017)

Specificity

Detects endogenous levels of total RhoGAP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

190 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RhoGAP Antibody (SR1304)

Western Blot: RhoGAP Antibody (SR1304) [NBP3-22307] -

Western Blot: RhoGAP Antibody (SR1304) [NBP3-22307] - Western blot analysis using NBP3-22307 in Hela cell lysate.

Applications for RhoGAP Antibody (SR1304)

Application
Recommended Usage

Flow Cytometry

1:50

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RhoGAP

The p190 Rho GTPase activating protein RhoGAP) family includes two closely related proteins, p190-A and p190-B. p190-B contains an N-terminal GTPase domain and a C-terminal Rho-GAP domain (1). p190-B can be directly phosphorylated on a single identified tyrosine residue by the activated insulin and IGF-1 receptors. This phosphorylation causes p190-B to translocate to the lipid rafts of the plasma membrane, where it can facilitate the inactivation of the Rho GTPase. P190-B also has been shown to be tyrosine phosphorylated by c-Src and v-Src. Additionall; the GAP domain of p190-B as shown to attenuate signal-transducing activity of Rac, Rho and CDC42 (2-3).

Alternate Names

growth factor independent 2, p100 RasGAP-associated p105 protein, p105 RhoGAP, p190-BGFI2, p190BRhoGAP, Rho GTPase activating protein 5, rho GTPase-activating protein 5, RhoGAP5, Rho-type GTPase-activating protein 5

Gene Symbol

ARHGAP5

Additional RhoGAP Products

Product Documents for RhoGAP Antibody (SR1304)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RhoGAP Antibody (SR1304)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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