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RhoGAP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20158

Novus Biologicals, part of Bio-Techne
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NBP2-20158

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for RhoGAP Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human RhoGAP. The exact sequence is proprietary.

Reactivity Notes

Mouse (82%).

Localization

Cytoplasm; Membrane; Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

172 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RhoGAP Antibody

Western Blot: RhoGAP Antibody [NBP2-20158]

Western Blot: RhoGAP Antibody [NBP2-20158]

Western Blot: RhoGAP Antibody [NBP2-20158] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE gel, diluted at 1:2000.
Immunohistochemistry-Paraffin: RhoGAP Antibody [NBP2-20158]

Immunohistochemistry-Paraffin: RhoGAP Antibody [NBP2-20158]

Immunohistochemistry-Paraffin: RhoGAP Antibody [NBP2-20158] - Immunohistochemical analysis of paraffin-embedded H441 xenograft, using antibody at 1:100 dilution.

Applications for RhoGAP Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RhoGAP

Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

growth factor independent 2, p100 RasGAP-associated p105 protein, p105 RhoGAP, p190-BGFI2, p190BRhoGAP, Rho GTPase activating protein 5, rho GTPase-activating protein 5, RhoGAP5, Rho-type GTPase-activating protein 5

Gene Symbol

ARHGAP5

UniProt

Additional RhoGAP Products

Product Documents for RhoGAP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RhoGAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.