Ribosomal Protein S6/RPS6 Antibody (4C7P4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15435
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4C7P4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human RPS6 (NP_001001.2). MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Ribosomal Protein S6/RPS6 Antibody (4C7P4)
![Western Blot: Ribosomal Protein S6/RPS6 Antibody (4C7P4) [NBP3-15435]](https://resources.bio-techne.com/images/products/Ribosomal-Protein-S6-RPS6-Antibody-4C7P4-Western-Blot-NBP3-15435-img0001.jpg "Western Blot: Ribosomal Protein S6/RPS6 Antibody (4C7P4) [NBP3-15435]")
Western Blot: Ribosomal Protein S6/RPS6 Antibody (4C7P4) [NBP3-15435]
Western Blot: Ribosomal Protein S6/RPS6 Antibody (4C7P4) [NBP3-15435] - Western blot analysis of extracts of various cell lines, using Ribosomal Protein S6/RPS6 antibody (NBP3-15435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
ICC/IF: Ribosomal Protein S6/RPS6 Antibody (4C7P4) [NBP3-15435] -
ICC/IF: Ribosomal Protein S6/RPS6 Antibody (4C7P4) [NBP3-15435] -Confocal imaging of NIH/3T3 cells using S6 Ribosomal Protein (RPS6) Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
ICC/IF: Ribosomal Protein S6/RPS6 Antibody (4C7P4) [NBP3-15435] -
ICC/IF: Ribosomal Protein S6/RPS6 Antibody (4C7P4) [NBP3-15435] -Confocal imaging of C6 cells using S6 Ribosomal Protein (RPS6) Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.Applications for Ribosomal Protein S6/RPS6 Antibody (4C7P4)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:100 - 1:2000
Western Blot
1:1000 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ribosomal Protein S6/RPS6
Alternate Names
RPS6
Gene Symbol
RPS6
Additional Ribosomal Protein S6/RPS6 Products
Product Documents for Ribosomal Protein S6/RPS6 Antibody (4C7P4)
Product Specific Notices for Ribosomal Protein S6/RPS6 Antibody (4C7P4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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