Ribosomal Protein S6/RPS6 [p Ser244] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29653

Novus Biologicals, part of Bio-Techne
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NBP2-29653
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (100%), Goat (100%), Guinea Pig (100%), Primate (100%), Rat (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ribosomal Protein S6/RPS6 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser244 conjugated to KLH. Accession # P62754

Modification

p Ser244

Specificity

Specific for endogenous levels of the ~28 kDa Ribosomal Protein S6/RPS6 phosphorylated at Ser244. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ribosomal Protein S6/RPS6 [p Ser244] Antibody

Western Blot: Ribosomal Protein S6/RPS6 [p Ser244] Antibody [NBP2-29653]

Western Blot: Ribosomal Protein S6/RPS6 [p Ser244] Antibody [NBP2-29653]

Western Blot: RPS6 [p Ser244] Antibody [NBP2-29653] - Jurkat cell lysate showing specific immunolabeling of the ~28k rpS6 phosphorylated at Ser244 (Control). Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen (peptide) but not by the corresponding dephosphopeptide (not shown).

Applications for Ribosomal Protein S6/RPS6 [p Ser244] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ribosomal Protein S6/RPS6

Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subunit that mediates translation initiation at the 5'-m7GpppG cap of mRNA. The rpS6 protein is both cytoplasmic and nuclear localized (Chen and Dittmer 2011). In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four Ser residues (Ser-235, Ser-236, Ser-240, and Ser-244) whose modification potentiates rpS6 cap binding activity (Hutchinson et al., 2011). Additionally, rpS6 phosphorylation and function are highly regulated and have been implicated in the regulation of translational initiation and protein synthesis in response to extracellular stimuli such as TRAIL and gamma interferon (IFN-gamma), as well as upon activation of the phosphatidylinositol 3-kinase (PI3K)-Akt-mTOR pathway (Chen and Dittmer 2011).

Alternate Names

RPS6

Entrez Gene IDs

20104 (Mouse)

Gene Symbol

RPS6

UniProt

P62754 (Mouse)

Additional Ribosomal Protein S6/RPS6 Products

Product Documents for Ribosomal Protein S6/RPS6 [p Ser244] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ribosomal Protein S6/RPS6 [p Ser244] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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