Rictor Antibody (7B3) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80925
![Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]](https://resources.bio-techne.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80925-img0007.jpg "Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]")
![Western Blot: Rictor Antibody (7B3)Azide and BSA Free [NBP2-80925]](https://resources.bio-techne.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Western-Blot-NBP2-80925-img0001.jpg "Western Blot: Rictor Antibody (7B3)Azide and BSA Free [NBP2-80925]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Primate
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 7B3
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Rictor Antibody (7B3) - Azide and BSA Free
Immunogen
Purified recombinant fragment of human RICTOR expressed in E. coli. [UniProt# Q6R327]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
192 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Rictor Antibody (7B3) - Azide and BSA Free
Western Blot: Rictor Antibody (7B3)Azide and BSA Free [NBP2-80925]
Western Blot: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysates. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]](https://resources.bio-techne.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80925-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]")
Immunocytochemistry/ Immunofluorescence: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]
Immunocytochemistry/Immunofluorescence: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Analysis of NIH/3T3 cells using RICTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]](https://resources.bio-techne.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80925-img0003.jpg "Immunohistochemistry-Paraffin: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]")
Immunohistochemistry-Paraffin: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]
Immunohistochemistry-Paraffin: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB staining. Image from the standard format of this antibody.![Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]](https://resources.bio-techne.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80925-img0004.jpg "Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]")
![Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]](https://resources.bio-techne.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80925-img0005.jpg "Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]")
![Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]](https://resources.bio-techne.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80925-img0006.jpg "Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]")
![Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]](https://resources.bio-techne.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80925-img0008.jpg "Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]")
Applications for Rictor Antibody (7B3) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1 ug per million cells
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Application Notes
This RICTOR (7B3) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA. The observed molecular weight of the protein in WB may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rictor
Long Name
Rapamycin-insensitive Companion of mTOR
Alternate Names
mAVO3, Pianissimo
Gene Symbol
RICTOR
Additional Rictor Products
Product Documents for Rictor Antibody (7B3) - Azide and BSA Free
Product Specific Notices for Rictor Antibody (7B3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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