Rictor Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-611
![Western Blot: Rictor Antibody [NB100-611]](https://resources.bio-techne.com/images/products/Rictor-Antibody-Western-Blot-NB100-611-img0003.jpg "Western Blot: Rictor Antibody [NB100-611]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Rapamycin-Insensitive Companion of mTOR using the numbering given in TrEMBL entry Q6R327 (GeneID 253260).
Specificity
NB100-611 is specific for human Rictor protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Rictor Antibody - BSA Free
Western Blot: Rictor Antibody [NB100-611]
Western Blot: Rictor Antibody [NB100-611] - Detection of Human Rictor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody NB100-611 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: Rictor Antibody [NB100-611]](https://resources.bio-techne.com/images/products/Rictor-Antibody-Immunoprecipitation-NB100-611-img0004.jpg "Immunoprecipitation: Rictor Antibody [NB100-611]")
Immunoprecipitation: Rictor Antibody [NB100-611]
Immunoprecipitation: Rictor Antibody [NB100-611] - Detection of human Rictor by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rictor antibody NB100-611 (lot 5) used for IP at 6 ug per reaction. Rictor was also immunoprecipitated by a previous lot of this antibody (lot 4) and rabbit anti-Rictor antibody NB100-612. For blotting immunoprecipitated Rictor, NB100-611 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for Rictor Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2- 10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Blots performed with NB 100-611 show additional bands not seen with NB 100-612.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Rictor
Long Name
Rapamycin-insensitive Companion of mTOR
Alternate Names
mAVO3, Pianissimo
Gene Symbol
RICTOR
UniProt
Additional Rictor Products
Product Documents for Rictor Antibody - BSA Free
Product Specific Notices for Rictor Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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