Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Rif1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Rap1 Interacting Factor 1 using the numbering given in entry NP_060621.3 (GeneID 55183).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rif1 Antibody

Immunohistochemistry: Rif1 Antibody [NB100-1585]

Immunohistochemistry: Rif1 Antibody [NB100-1585]

Immunohistochemistry: Rif1 Antibody [NB100-1585] - Sample: FFPE section of human laryngeal squamous cell carcinoma. Antibody: Affinity purified rabbit anti- RIF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: Rif1 Antibody [NB100-1585]

Immunoprecipitation: Rif1 Antibody [NB100-1585]

Immunoprecipitation: Rif1 Antibody [NB100-1585] - Detection of Human RIF1 on HeLa whole cell lysate using NB100-1585. RIF1 was also immunoprecipitated using rabbit anti-RIF1 antibodies NB100-1586 and NB100-1587.

Applications for Rif1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

1-5 ug/mg of lysate
Application Notes
Western blot: Not recommended. Use rabbit anti-RIF1 antibodies NB 100-1586 or NB 100-1587. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Rif1

RAP1 interacting factor (RIF1) localizes to dysfunctional telomeres and functions in the S-phase checkpoint. In response to double-stranded breaks (DSBs), RIF1 functions in the ATM and ATR mediated response to DNA damage. RIF1 foci formation in response to DNA damage is completely dependent on ATM. Alternate names for RIF1 include telomere-associated protein RIF1, Rap1-interacting factor 1 homolog, FLJ12870, and DKFZp781N1478.

Alternate Names

DKFZp434D1026, DKFZp781N1478, FLJ10599, FLJ12870, RAP1 interacting factor homolog (yeast), Rap1-interacting factor 1 homolog, telomere-associated protein RIF1

Entrez Gene IDs

55183 (Human)

Gene Symbol

RIF1

UniProt

NP_060621.3 (Human)

Additional Rif1 Products

Product Documents for Rif1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rif1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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