Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Rif1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1425 and 1450 of human Rap1 Interacting Factor 1 using the numbering given in entry NP_060621.3 (GeneID 55183).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rif1 Antibody

Western Blot: Rif1 Antibody [NB100-1586]

Western Blot: Rif1 Antibody [NB100-1586]

Western Blot: Rif1 Antibody [NB100-1586] - Detection of Human RIF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RIF1 antibody NB100-1586 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: Rif1 Antibody [NB100-1586]

Immunohistochemistry-Paraffin: Rif1 Antibody [NB100-1586]

Immunohistochemistry-Paraffin: Rif1 Antibody [NB100-1586] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RIF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: Rif1 Antibody [NB100-1586]

Immunoprecipitation: Rif1 Antibody [NB100-1586]

Immunoprecipitation: Rif1 Antibody [NB100-1586] - Whole cell lysate from HeLa cells. Whole cell lysate from 293T cells. Affinity purified rabbit anti-RIF1 antibody NB100-1586. RIF1 was also IPed using rabbit anti-RIF1 antibody NB100-1587.
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Applications for Rif1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunoprecipitation

1-5 ug/mg of lysate

Western Blot

1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Rif1

RAP1 interacting factor (RIF1) localizes to dysfunctional telomeres and functions in the S-phase checkpoint. In response to double-stranded breaks (DSBs), RIF1 functions in the ATM and ATR mediated response to DNA damage. RIF1 foci formation in response to DNA damage is completely dependent on ATM. Alternate names for RIF1 include telomere-associated protein RIF1, Rap1-interacting factor 1 homolog, FLJ12870, and DKFZp781N1478.

Alternate Names

DKFZp434D1026, DKFZp781N1478, FLJ10599, FLJ12870, RAP1 interacting factor homolog (yeast), Rap1-interacting factor 1 homolog, telomere-associated protein RIF1

Entrez Gene IDs

55183 (Human)

Gene Symbol

RIF1

UniProt

Q5UIP0 (Human)

Additional Rif1 Products

Product Documents for Rif1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rif1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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