Rif1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-973

Novus Biologicals, part of Bio-Techne
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NB300-973
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Rif1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CEEGIIDANKTETNT corresponding to internal region according to NP_060621.3.

Specificity

This antibody is expected to recognise an epitope corresponding to aa 2076-2089 of human RIF1 protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rif1 Antibody

Immunohistochemistry-Paraffin: Rif1 Antibody [NB300-973]

Immunohistochemistry-Paraffin: Rif1 Antibody [NB300-973]

Immunohistochemistry-Paraffin: Rif1 Antibody [NB300-973] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Rif1 Antibody [NB300-973]

Immunohistochemistry-Paraffin: Rif1 Antibody [NB300-973]

Immunohistochemistry-Paraffin: Rif1 Antibody [NB300-973] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Rif1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000
Application Notes
WB: Not yet tested - Our standard WB protocol would not expect to reliably detect proteins as large as the predicted MW of approx. 247 kDa band according to NP_060621.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rif1

Rif1 (Rap1-interacting factor homolog) is a protein whose primary function is in the DNA-damage response, RIF1 forms foci that colocalize with other DNA damage response factors, after induction of double-strand breaks. Rif1 localizes to double strand breaks in an ATM (ataxia telangiectasia mutated)- and 53BP1-dependent manner and functions in the intra-S-phase checkpoint that serves to slow down DNA synthesis when DNA damage has occurred.

Alternate Names

DKFZp434D1026, DKFZp781N1478, FLJ10599, FLJ12870, RAP1 interacting factor homolog (yeast), Rap1-interacting factor 1 homolog, telomere-associated protein RIF1

Entrez Gene IDs

55183 (Human)

Gene Symbol

RIF1

UniProt

NP_060621 (Human)

Additional Rif1 Products

Product Documents for Rif1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rif1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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