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Rif1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26129

Novus Biologicals, part of Bio-Techne
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NBP2-26129

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-RNLQSRWRSPSHEN corresponding to C-Terminus according to NP_060621.3, NP_001171134.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_060621.3; NP_001171134.1). The immunizing peptide sequence has been optimised for cross-reactiviy in mouse. Reported variants represent identical protein: NP_001171135.1, NP_001171136.1, NP_001171134.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rif1 Antibody

Immunocytochemistry/ Immunofluorescence: Rif1 Antibody [NBP2-26129]

Immunocytochemistry/ Immunofluorescence: Rif1 Antibody [NBP2-26129]

Immunocytochemistry/Immunofluorescence: Rif1 Antibody [NBP2-26129] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunocytochemistry/ Immunofluorescence: Rif1 Antibody [NBP2-26129]

Immunocytochemistry/ Immunofluorescence: Rif1 Antibody [NBP2-26129]

Immunocytochemistry/Immunofluorescence: Rif1 Antibody [NBP2-26129] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Rif1 Antibody

Immunocytochemistry/Immunofluorescence: Rif1 Antibody [NBP2-26129] -

Immunocytochemistry/Immunofluorescence: Rif1 Antibody [NBP2-26129] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for Rif1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:128000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rif1

Rif1 (Rap1-interacting factor homolog) is a protein whose primary function is in the DNA-damage response, RIF1 forms foci that colocalize with other DNA damage response factors, after induction of double-strand breaks. Rif1 localizes to double strand breaks in an ATM (ataxia telangiectasia mutated)- and 53BP1-dependent manner and functions in the intra-S-phase checkpoint that serves to slow down DNA synthesis when DNA damage has occurred.

Alternate Names

DKFZp434D1026, DKFZp781N1478, FLJ10599, FLJ12870, RAP1 interacting factor homolog (yeast), Rap1-interacting factor 1 homolog, telomere-associated protein RIF1

Entrez Gene IDs

55183 (Human); 51869 (Mouse)

Gene Symbol

RIF1

Additional Rif1 Products

Product Documents for Rif1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rif1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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