Rif1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47303

Novus Biologicals, part of Bio-Techne
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NBP2-47303
NBP2-47303-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rif1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ELNVGNEASFHGQERTKTGISEEAAIEENKRNDDSEADTAKLNAKEVATEEFNSDISLSDNTTPVKLNAQTEISEQTAAGELDGGNDVSDLHSS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rif1 Antibody

Immunocytochemistry/ Immunofluorescence: Rif1 Antibody [NBP2-47303]

Immunocytochemistry/ Immunofluorescence: Rif1 Antibody [NBP2-47303]

Immunocytochemistry/Immunofluorescence: Rif1 Antibody [NBP2-47303] - Identification of an HDR-enhancing fragment of 53BP1. Representative IF images showing HA-tagged DN1 or DN1S and RIF1 recruitment to IR-induced DNA repair foci. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-10735-7), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Rif1 Antibody [NBP2-47303]

Immunohistochemistry-Paraffin: Rif1 Antibody [NBP2-47303]

Immunohistochemistry-Paraffin: Rif1 Antibody [NBP2-47303] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.
Immunocytochemistry/ Immunofluorescence: Rif1 Antibody [NBP2-47303]

Immunocytochemistry/ Immunofluorescence: Rif1 Antibody [NBP2-47303]

Immunocytochemistry/Immunofluorescence: Rif1 Antibody [NBP2-47303] - Staining human cell line HEK 293 shows positivity in nucleus. Antibody staining is shown in green.

Applications for Rif1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rif1

Rif1 (Rap1-interacting factor homolog) is a protein whose primary function is in the DNA-damage response, RIF1 forms foci that colocalize with other DNA damage response factors, after induction of double-strand breaks. Rif1 localizes to double strand breaks in an ATM (ataxia telangiectasia mutated)- and 53BP1-dependent manner and functions in the intra-S-phase checkpoint that serves to slow down DNA synthesis when DNA damage has occurred.

Alternate Names

DKFZp434D1026, DKFZp781N1478, FLJ10599, FLJ12870, RAP1 interacting factor homolog (yeast), Rap1-interacting factor 1 homolog, telomere-associated protein RIF1

Gene Symbol

RIF1

Additional Rif1 Products

Product Documents for Rif1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rif1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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